When analyzing the above picture the red/green represents cell type and a protein. The problem is that in the white circles (in the circular area with no nuclei)there are some background colors that are included as cell type and protein even if this should not be possible. It must be background.
My question is: Is there a way to limit the inclusion to red/green signal so that they have to be within XX pixels from a nuclei. The cells i’m looking at are T-cells which mainly are just nuclei with a cytoplasm very close to these.
Does the above explaination make any sense?