How to do dual-excitation ratiometric analysis in ImageJ?

Hello, guys. I’m using a fluorescent protein called mkeima with dual excitation but single emission spectrum(ex438-em610 / ex543-em610). I want to use imageJ to calculate the ratio of signal(610) from 543 to that from 438. How can I do this? Thanks.

Hi @carrierka

This section of the wiki may be of help:

Best
jy

1 Like

Tinevez, thanks. Unfortunately, that plugin has been remove from uhnresearch. IF anyone has it, please contact me here or through my email ka_luo1202@hotmail.com.

Hi @carrierka

Yes, you need to subscribe to the Update site that contain the plugin. Everything is explained here:

Also, please restrain from requesting info via email. If we make this information public, it can searched and found by anyone in the future.

1 Like