Dear members, I do have pictures of membrane bundles. I would like to calculate the surface of the membranes. It is to say, all fibers are cut (see attachment) and I want to calculate the surface of each fiber.
The picture is from a micro-CT in Tiff format. I first improve brightness contrast then I make it an 8 bit image. Then I set a threshold but then I start to get troubles. I can not mask only the internal lumen of the fiber so counting the number of fibers and calculating their surface is not possible. For those interested the number of fibers should be close to 2000.
Any help from this experienced forum would be more than appreciated. As I am just starting with Fiji ImageJ, please be patient with me.
topTest_0934.tif (366.1 KB)
Thanks in advance