Sample image and/or code
Hello, I’ve got a lot of images of beta cell islets surrounded by isolated single cells. I’m trying to make a pixel classifier to quickly identify those single cells and count them, but I’m running into some trouble. I tried making a stock pixel classifier using qupaths pixel classifier tool (random tree), but it has a relatively high false positive and negative rate. I imagine something that can identify cells, then check the surrounding area for more cells would do the trick, but I’m not sure how to go about this.
If you’ve made it this far, I really appreciate your time. Let me know what you think.
Have a good day!