Is it possible to convert intensity color scale to concentration color scale in images in ImageJ? For example, I have 8-bit image. I opened it in ImageJ and created color scale using Analyze–>Tools–>Calibration Bar. It created color scale with range 0…255. Is it possible to convert this color scale to mmol/liter color scale, for example 0…150 mmol/liter?
I will be very appreciate for any help.
You can use the analyze -> Calibrate tool to perform this conversion.
If it’s a simple function and you just need the values, you can also convert the image to 32 bit, then use one of the Process->Math functions to make a new image.