Hi! Basically I want to do what is shown in the drawing.
I acquired multiple individual tilescans all from a single slide, and they are all wrapped up in a single .lif file.
My plan was to import the.lif file with BioFormats, and use the ‘concatenate series when compatible’ option to put all 3-channel images in a single stack as if they were a time series.
Followed by Image > Stacks > Make montage in order to put them all together in a single image.
But the concatenate series when compatible option only combines different tilescans (along the t axis) when their dimensions are the same, which is not the case here. Each tilescan has a different aspect ratio and size.
Further, make montage only keeps one channel, so I’d have to do this individually for many channels.
As you can imagine, I have dozens of these slides (.lif files) and each of them have ~20 tilescans.
Surely there must be an easier way to do this?