We stained the monocytes with RGB colors, blue for nucleus, red for cytoplasm and green is the protein of interest. We want to compare the area among red, green and blue, to see the migration of the green from cytoplasm to nucleus.
For example, I use analyze>analyze particles to automatically select the cells I want in blue (first pic), then I go to the green and select the same spot/cell and use the wand to select the green area and add to the ROI manager. For this cell particularly (second pic), I can see the blue as in #8 and green as in #89. I can just measure the area manually by clicking measure but I really want to do it automatically. How do I let the program know #8 and #89 is together? so that it can output the measurement together? And output them for example, first column is the area of #8 and the second column is #89?