I have some .swc tracings that I import to the ROI manager in ImageJ through the Simple Neurite Tracer (SNT) plugin.
The problem that I am facing is that these ROI’s are an open polygon,
And I want to use the Analyze particles plugin only on the area inside this tracing (after thresholding, the analyze particles workflow is already made). Is there a way to modify a ROI so that it gets “closed” via the shortest possible path and that it is recognized as a closed, not an open ROI?
Currently, when I try to Analyze particles it analyzes the whole picture.
Thank you for your help,