I have tried to understand how splitting channels works but it still remains unclear for me. I am analyzing COX and SDH activity in mouse muscle. One colleague taught me to use ImageJ but she was unaware why she had done the things she did with ImageJ. She said I should split channels before analyzing the image.
How do know what channel to use? Is it staining-dependent or what? Can I use the same calibration for all channels?
I have read the manual and several topics here in the forum but couldn’t find an answer…
Thank you for all the help!