How to calculate the protein expression on brain section. I tried with TrakEM2 but without good results. Is there a specific plugin?

I want to quantify the expression of a protein in healthy and AD neurons. I see on the IHC images that the protein of interest is clearly decreased in AD as compared to controls, but I cannot “translate” this with statistics. Using the right software will help me to obtain a significant difference.

Thank you,

Emanuele

IHC is does not involve stoichiometric reactions, and worse, uses number of amplification steps, so the amount of product is not linearly related to the amount of antigen. No software will allow to find the answer you are after and make comparisons across samples. In general, people use IHC more like a Yes/No evidence of expression at a certain primary antibody concentration.

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