How to calculate intensity of a ROI in moving C.elegans with time series?

Dear Friends,
Can anyone please give me suggestions about the following problem?
I immobilized C.elegans but still, it is moving slightly during my time-series experiment. I want to measure the intensity of an ROI in the C.elegans? How can I measure the mean intensity of the moving worm? Please suggest.

Thank you.
with kind regards,

Hello Nirnay,

You could try “TrackObject” and “MeasureObjectIntensity” modules.
You may find the object tracking example helpful.

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Dear Nasim,
Thank you very much for your suggestion. I will try accordingly!