How to calculate diameter of multiple particle by using macro in ImageJ?

[Praveen_kumar_R]

I want to measure the diameter of each one. is there any macro or plugin for this?

[etadobson (Ellen TA Dobson)]

Hey @Praveen_kumar_R

Welcome to the Forum!

So to start scripting with ImageJ/Fiji… here are few helpful links to get you started:

• ImageJ Wiki - the go-to place to search for help with ImageJ/Fiji
• Scripting overview page on the wiki
• A helpful workshop on Scripting with Fiji - the corresponding slides are here
• Introduction into Macro Programming page of the wiki
• Built-In Macro Functions list
• An overview of the ImageJ Macro language - also on that page is a section on functions

Here is a little macro I put together that might get you what you need…

setOption("BlackBackground", true);
run("Make Binary");
run("Analyze Particles...", "size=200-Infinity exclude clear add"); // you can set different size limits here if you want

count = roiManager("count"); 

for (i = 0; i < count; i++) {
	roiManager("Select", i);
	run("Fit Ellipse");
	roiManager("Add");
	roiManager("Select", i);
	roiManager("Delete");
}

roiManager("Deselect");
roiManager("multi-measure measure_all");

You can change what is measured in Set Measurements.

Hope this helps!

eta

[Praveen_kumar_R]

setOption(“BlackBackground”, true);
run(“Make Binary”);
run(“Analyze Particles…”, “size=200-Infinity exclude clear add”); // you can set different size limits here if you want

count = roiManager(“count”);

for (i = 0; i < count; i++) {
roiManager(“Select”, i);
run(“Fit Ellipse”);
roiManager(“Add”);
roiManager(“Select”, i);
roiManager(“Delete”);
}

roiManager(“Deselect”);
roiManager(“multi-measure measure_all”);

Thanks alot…