I have got some images of transparent gel bead. Because it is transparent, I cannot turn it into masked Black&white image using “8-bit->threshold->set measurements (Feret’s Diameter)”.
is there another way to obtain the outline and turn it into a solid black&white image for diameter analysis?
Alternatively, is there a macro script/plugin that are designed for such transparent praticle?
in some previous images, the gel beads are overlap with each other. How to process the overlap images for the diameter analysis in batch?