I have a two-channel timelapse dataset depicting a single unidirectionally growing cell.
The data is arranged in two separate stacks (transmission and fluorescence).
I would like to align all slices in the fluorescence stack, but can’t use classical registration/template matching tools due to the lack of clear recurrent features in the fluorescence images.
To solve this, I’m using template matching to align all slices in the transmission image stack, and extract the new X/Y coordinates for each slice.
I would now like to apply those coordinates to the fluorescence stack.
However, until now I haven’t found a way to do this.
So my questions is: How to apply slice alignment coordinates of one stack to another?
Any great ideas?