I performed a coculture experiment in which my cells of interest have red nuclei. I want to determine the percentage of those nuclei that are also green and/or blue.
On FIJI I would use a watershed segmentation, threshold my 3 channels, create a mask in the red channel, transfer it to the green and blue channels and count the number of particles in each channel.
How do I translate this in CP? Should I “IdentifyPrimaryObjects” in each channel and then use the “RelateObjects” module, once with the green objects as child objects and once with the blue ones and child objects? I guess this would work for the Red+Green+ and Red+Blue+ populations, but how to I obtain the Red+Green+Blue+ population?
Is there anything I should be careful about? Any downstream modules I should use?
Thanks for your help,