I am a biology Ph.D candidate and I have a problem when working on my stuy recently. I want to analyze the fluorescence intensity of different region in a single picture. I have searched the ImageJ website, but I did not find the method and plugins to analyze the fluorescence intensity of a single picture. I noticed that there are only colocaliztion analysis and intensity vs time analysis. Is there anybody konw how to analyze the fluorescence intensity of single picture. I will deeply appreciate.
Hi @xinke -
Welcome to the Forum!
Would you be able to post an example image (preferably a raw dataset) and specifically explain what you want to measure. ‘Analyze fluorescence intensity of a single picture’ is a bit broad - so if you can help guide us with exactly what you want to measure - we can help show you how to do it using ImageJ.
Thanks very much for your reply. Here is my problem. I want to measure the fluorescence intensity of the cell membrane and intracellular respectively in the picture as below .
If you known the method, please tell me how to deal with it. I am a new fish about ImageJ and I am trying hard to work on it. Thanks for your suggestion.
Thank you once again.