I have images from a time-lapse microscopy experiment (multiple channels and multiple time points). Unfortunately, due to microscope drift the consecutive time points are not exactly at the same XY position (slight systematic shifts between time points).
- I would like to use the align function to iterate over all time points and sequentially re-align this shift.
- Since this I have images from multiple channels, I would like to use only one channel (the one with tagged nuclei) to calculate the drift and then propagate the shift and crop values to other channels.
Could this be done with the align function or any other module?
PS - I’ve been using CellProfiler for a almost a month and am finding it extremely useful (thanks for the great work)