I am new to Cell profiler and am trying to get the collected perimeter and area data in um, not pixels. Any advice on how to do this?
Also, my pipeline often segments the cells where segmentation should not occur, leading to a higher cell count. Is the only solution editing my images prior to running the program?
Attached are the images of the bad segmentation & area/perimeter output. I’ve also attached the pipeline I’ve haphazardly created, which isn’t exactly working.
Thanks in advance for any support!
bb_pipeline4.0.cpproj (455.4 KB)