I actually started using Cell Profiler and might need some help.
I want to analyze 16-bit tiff images so I create the following pipeline:
Everytime I load 16-bit images I get an nonsense results even if I uncheck “Rescale intensities”.
If I use 8-bit images is works fine, but for future analysis (measuring intensity, size, …) I want to use the original image type.
Thanks a lot for your help,
I used a Nikon Eclipse Ti inverted microscope
I also got the same error using images from a Leika Confocal microscope.
I allready installed the programm new and tried it again, but this leads to the same result.