Hello CPForum members,
I would like to know how CellProfiler (CP) measures “Nuclei_Granularity_5_Mito float”
I am doing Cell Paint assay with attached pipeline and using CP (My CP version is 2.2.0).
final_analysis.cppipe (41.4 KB)
I read this post and I understand the feature value called “Granularity” in CP is the distribution of granularity and the number like “_5” mean the size of object.
Then, I think “Nuclei_Granularity_5_Mito float” means the granularity distribution of “Mitochondria” whose size is “5” in the region of nucleus (the region of nucleus is determined with “IdentifyPrimaryObjects” module).
I don’t understand the meaning of mitochondria in a nucleus from the view of biology because there is no mitochondria in a nucleus, but is my understanding for the feature value called " Nuclei_Granularity_5_Mito float" right?