How do I convert pixels into µm in cellprofiler program?

I have been calculating area in pixels but that is not it should be represented while publishing an article. Can anyone help me with how I can use the program to convert from pixels to µm. Thank you.


Hi Nikhil,

Generally, Cellprofiler gives in pixels. The pixel to micrometer conversion you have to do it. Unfortunately, program doesn’t do. Useful old links,

Fujifilm Wako Automation (Consultant)

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If you know your own pixel-to-micrometer conversion factor, you can multiply it directly times any 1D measurements (radius, etc) and multiply it squared times any 2D measurements (area, etc)- you can either do this yourself after-the-fact, or in the CalculateMath module.


Thank you very much bcimini.