I have a stack of images of cells with both phase and fluorescence images. With the phase images, I am able to use Find Maxima and Analyze Particles to extract an outline of each cell in each frame into the ROI Manager. I would then like to get the fluorescence intensities in each frame from these ROIs. If I use Measure in the ROI Manager, I get a big list of intensities with no information on which frame it came from. If I run Multi-Measure, it measures each ROI in every single frame, not only in its native frame.
Is there a straightforward way to get an organized table of fluorescence intensities using ROIs, which includes information on which frame each measurement came from? I do not need to track the cells, only extract their fluorescence from each frame.
Thanks for any help!