Good evening everyone,
I want to analyze the speed of Paramecium caudatum cells under various conditions. I use Fiji and the plugin TrackMate (more details: LoG detector, Simple LAP tracker). After having solved the usual problems, the program produces a lot of data after the Analysis step. One file contains among other data the “TRACK_MEAN_SPEED” which to me sounds like the speed of a given track. However, there is an inverse relation between the speed and the number of analyzed spots / track duration. So the longer the track duration, the lower the speed - which doesn’t make any sense. If you have a solution / advice, I would very much appreciate it. Thanks!