Hi all,I’m new to the forum !
I have a microscope fluorescence image of which the background is not perfectly black. Is there a way I can pick a spot on the image and “tell” ImageJ/FijIJ that it should define this as perfect black, thus adjusting the colours of the entire image the way you can do it in Photoshop ?
I’d be very grateful if anyone could help me. I have tried to search for information on Google and also tried out a lot of functions, but so far I haven’t succeeded. I am using ImageJ 1.50b with the FiJI plugin, if that is of any use.