How can I do quantification for Green fluorescent cells!?

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How can I do quantification for Green fluorescent cells!?

Can you be more specific here? What exactly do you want to quantify?

  • The number of positive cells? (If so, which cells do you consider positive?)
  • The total amount of fluorescence signal in the entire image?
  • The average amount of fluorescence signal per cell? (Including or excluding the nucleus?)

For some basic concepts, I recommend reading the following sections in the user guide:

sorry for my short unspecified words,Im new here it is my first question still checking how and where to use my question…I know there are my programs for images analysis I tried to use one of them but I got lost so im here to ask …Im thankful for your reply…

Could anyone write me the steps for quantification of positive GFP signal because I couldn’t know how to get the best threshold for the photo and how to separate attached two cells

https://theolb.readthedocs.io/en/latest/imaging/measuring-cell-fluorescence-using-imagej.html