How can I do quantification for Green fluorescent cells!?
Can you be more specific here? What exactly do you want to quantify?
- The number of positive cells? (If so, which cells do you consider positive?)
- The total amount of fluorescence signal in the entire image?
- The average amount of fluorescence signal per cell? (Including or excluding the nucleus?)
For some basic concepts, I recommend reading the following sections in the user guide:
sorry for my short unspecified words,Im new here it is my first question still checking how and where to use my question…I know there are my programs for images analysis I tried to use one of them but I got lost so im here to ask …Im thankful for your reply…
Could anyone write me the steps for quantification of positive GFP signal because I couldn’t know how to get the best threshold for the photo and how to separate attached two cells