I am a student and research the topic of the critical cranial bone defect.
Now, I am confused with a problem that I used Bone J to calculate the BV/TV ratio of the defect site.
But the data showed higher than I except (I except the negative control will approach to 0%, but I obtain 95%). So I would wonder to know if I set the wrong parameter during the process, or I need to set something new?
This is my setting process:
Specify for a region → Adjust the new Contrast → Duplicate the stack would like to calculate → Set the Threshold to convert Binary → Plugin (→Bone J→Fraction→Area/Volume fraction)
In addition, I also can’t obtain the same total volume after specifying the same region. How to explain this problem? Does the result decide to the head position of Rattus during scanning?
Thanks for the person who resolves my problem. If there has the wrong grammar, please correct to me!