How can I analyze three image sets using cellprofiler?

Hi, I’m totally a new learner, but I’m in a hurry to ask this question, I wanna differentiate two cells using machine learning methods attached to CPA. I have three image sets, two types of pure cells and a mixed one. I want to train machine with two pure cells and score the mixed one. How can I use CP to analyze those image sets in only one pipleline. Now I have three pipelines analyzing three cells, but I find it difficult to combine them into a one pipeline cuz I can not start CPA with three property files. Really looking forward to your reply, thank you! It is very urgent to solve this problem, thanks again.


It is possible to detect all three cell population in one CP pipeline. ClassifyObjects module can be employed to define different classes based on the defined measurements. Did you try this option?