Hello! I would like to measure the fluorescence intensity into each cell starting from the nucleus to the cell peryphery, because I am interested to measure the distribution of my protein from the peri-nuclear area to the cell periphery after some drug treatments. I have already seen this in a paper in which the authors drawn a rectangle which start from the nucleus and ends to the cell plasma membrane, then they divided this rectangle in differet little rectangles and measured “the distribution of gray values as percent of total intensity on a 100-pixel-long axis, where zero is the end of the nucleus and 100 is the end of the cell” (as they wrote in the paper), like that they got a graphic with a curve if there is a polarization of the signal into the cell (more close to the nucleus and no signal at the periphery) or a streght line if the signal is omogeneous along the rectangle.
Can you help me? I am not so good with ImageJ, so if you could give me the details I would be greatful.