I’m trying to recreate a neat visualization (+ subsequent segmentation for quantification) of the following 3D dataset (“side view”+ “top view”) using Imaris. Since I was afraid to bleach my signal (B220-AF647) I used a very high gain and therefore collected along a lot of background. However, we can clearly distinguish the signals from the background.
I tried Background Removal (“After processing”) and then to segmentate (“Segmentation”). However, I am not entirely satisfied with the end result. How would you proceed? I also tried Deconvolution but the difference is not very high. Thank you in advance for your inputs!