I am new at ImageJ processing, therefore my lack of knowledge leads me to this forum.
Getting to the point is that I need to run particle counting and sizing on starch particles, however my images often have overlapping starch particles and watershedding won’t help separating each particle. I’ve tried some online tutorial like gaussian blurring but to no avail. Also tried to do it manually but a single picture took me more than 30 minutes (I am not really familiar about imageJ options and functionality).
The data that i need to extract from the image are total particle numbers, particle diameters, number of occurring particle from each range (Very small: <5um ;Small:5-10um ;Medium:10-25um ;Large:>25um).
I have no prior knowledge about programming and not sure if i can run an automated analysis on my samples and the overlapping particles is a problem.
Many thanks if anyone can help me.