Dear to Whom it may concern,
I am conducting research with my professor, Dr. Al Mikell, at Oklahoma Christian University in reference to total heterotrophic bacteria examination in non-potable pond waters for our water filtration research project. Our particular research is geared to making water filtration cheaper and more effective for third world countries. The specification of my role within this project is examination of variation of diluents in reference to dilution efficacy comparable to standards. Beyond actually counting of cells , we would like to examine colony morphology and physical characteristics for qualitative and quantitative comparisons of varying CFUs (colony forming units). I examined the various pipelines and had difficulty in actually being able to create a software that could have all of the needed specifications. I saw that the yeast patch and yeast colonies examples given via your webstite, and they were similar to what I need if they were combined together. I was at a loss to come to conclusion of the method to do so, however, thus posting my concern here. I also am trying to be able to differentiate within a higher variation of colors (purples,blues,greens,yellow,whites, oranges) than those used within these examples. There are other traits that determine the colony morphology such as surface gleen, opacity, and shape, which would be helpful in determination, but understood if unable to differentiate within this program. I have attached two picture of a couple of the plates that I am attempting to be able to count. Thank you for reading this and considering to help. Hope you have a great day!