I want to measure the area of the different cells on this picture but I’m a bit stuck. Any help please. 001.tif (5.5 MB)
Try: Analyze particles…
Before you may want to apply a color threshold to select your cells…
Here are some super helpful links to get you started with ImageJ:
- ImageJ wiki - the best place to learn everything about ImageJ/Fiji!!
- “Introduction to Fiji” workshop and corresponding slides- worth the time to get a solid intro (more recently updated slides are here)
- Principles page - collection of principles for the entire image analysis process, from acquisition to processing to analysis
- Segmentation page
- “Segmentation in Fiji” workshop and corresponding slides (more recently updated slides are here)
- Trainable Weka Segmentation (TWS) plugin and it is scriptable via macro code - a great tool for segmentation that comes directly with Fiji. NOTE: Fiji is Just ImageJ - it is simply a distribution of ImageJ that comes with a bunch of plugins bundled - ready for you to use out-of-the-box. If you are just getting started, we recommend downloading/using Fiji.
- MorphoLibJ plugin - another great tool for segmentation that comes with Fiji, providing more options/techniques… comes with Morphological Segmentation tool
Using the TWS plugin I linked above… I was able to get decent segmentation of your cells (using default settings - so could be refined… also improved with some pre-processing steps before using TWS):