I use CellProfiler to quantitate individual cell sizes in tissues. I am thankful to you guys, since CP works best so far among softwares that I have tested for single cell quantifications. However, still have some false positive data points (due to overlapped cells, false particles from fragmented cells etc).
I thought CPA can help me to efficiently sort out false data points. I really want to try it out (I even played with CPA examples), however, learning CPA isn’t as intuitive as CP for me. I want to see scatter plots or data distributions of all data points to locate outliers. Honestly I don’t know how to start with. I have already generated data spreadsheet with CP. Ideally, I want to see scatter or histogram plots of my data set next to original images with labeled identified particles. This would help me to determine outliers with great confidence.