I am trying to track cells during a time lapse experiment, with an emphasis on correctly identifying cell divisions and cell deaths. I hope to use LAP, since it seems to be designed to identify such events accurately. I am assuming that a good 1st pass is important, but I am not getting great results so far. The problem is that objects that seem to be clearly trackable in subsequent frames are getting new object numbers in the 1st pass. For example, in the attached sample images, note the cluster of 4 cells on the right that have #'s 9,11,15,12 in Frames 1-2 and then #'s 29,31,33,34 in Frame 3. I have tried increasing the search radius from the default, and this has helped a bit, but not as much as I would like. Also, I don’t know whether I should be changing the search radius using the SD multiplier or the min/max thresholds or both.
As an alternative, the overlap and distance methods work fairly well, but I am concerned that I still may need to do too much manual review of splits and new/lost object calls.