First, thanks to all of the CP team for making a great product freely available and for the time you spend answering questions on how to use it! You are doing all of us who struggle to get numbers from images a great service!
I need some help with a pipeline and in interpreting the spreadsheets it produces. I have a project where I have a bacterium that expresses two fluorochromes - mCherry is constitutively expressed and GFP is inducibly expressed. My goal is to understand protein expression dynamics at the single-cell bacterium level by comparing GFP expression against mCherry expression. In the end, I want to be able to compare the intensity of each bacterium’s red and green signal, as well as the number of red and green pixels per bacterium (each bacterium is identified by its mCherry signal). It seems to me that this is an AND-type operation, as in, analyze pixels that are red AND green, but I’m not sure my pipeline is actually doing this, or which metric in the .csv file is the appropriate measurement for my analysis.
Can you help me by looking at my pipeline, and letting me know how to interpret the spreadsheet for my purposes? I have attached my pipeline and a representative image.
Thanks for your time and attention!
mCherry-GFP expression analysis.cpproj (408 KB)