I was just about to start using the TANGO plugin for segmentation of my image stack data. However, with the template processing chain provided from the TANGO website the plugin misses most of the nuclei in my data and is not very helpful for now.
I was wondering how sensitive TANGO is for out-of-focus signal , since I acquired the images in a way that there is still some diffracted signal at the bottom and top of the stack…?
Also, does anybody know about some tutorials/documentations or even pre-set processing chains available for download that can help getting started with TANGO? Currently, I find it difficult to set up processing chains that make sense and fit to my data without any comparison or idea which combinations make sense…
Thank you and best wishes,