I’ve been trying to figure out how to obtain the average intensity of the channel 2 using the area identified by channel 1. I am sort of a beginner and have searched the forums for help but haven’t figured it out completely.
I used automatic thresholding and a size cutoff of 10 to 200 but there are some faint dots that it is not picking up in channel 1. So I haven’t gotten past this stage. I then would assume that I have to use the outliens for this image to get the intensities from the second channel? If so, what is the best way? Mask? Thanks!
For some reason, it is not letting me upload my files. Are they too big? They are 1.6 MB TIff images