I’m new to CellProfiler and I’m investigating its capabilities with some images from our lab.
I understood the organic of Cell Profiler Software, but the difficult part looks to be the customization of each module to our Images (and also try to define a standard method of acquisition).
I’m interested to perform live Human Cell Image Segmentation.
I runed your Example Pipeline for human cells in our images, but it didn’t work so well. I think, probably I’ll need some smooth filter or maybe not. Please take a look.
The original Image
Here are the results with your pipeline.
The big change happens when I changed the typical diameter of objects in Module “IdentifyPrimaryObjects”. It Identifies to many edges in both cells and nucleus.
I’m using a range of 80, 130 pixels for nuclei objects.
test3.cp (8.65 KB)
Results.zip (1.37 MB)