I am new to Cell Profiler, and I am trying to work with tissue samples. I am trying to count cells that express CD68(green) and SM22alpha(red) but I am having trouble detecting the double positive cells(i think its because of the CD68 staining). The other issue that I am facing is with the “ExportToSpreadsheets” module. I keep getting a pop-up that says"Data not written to spreadsheets in test mode". I have attached my pipeline here.
SmoothMuscleCells.cpproj (762.3 KB)