Hello,
I am running this exact pipeline https://github.com/CellProfiler/tutorials/blob/master/3d_monolayer/3d_monolayer_final.cppipe
using these images
3d_2wkrpapsesg_xy1_ch2.tif (10.2 MB) 3d_2wkrpapsesg_xy1_ch1.tif (10.2 MB)
And I haven’t been able to run it to completion because I keep getting an error on step imagemath#22 that says error the image sizes don’t line up and to use the crop or align tools but I have followed the rest exactly and the align tool doesn’t work on 3D analysis. I’m at a dead end and not sure how to fix this, any help would be really really appreciated. Thanks.