Help using NIS .nd2 z stack files with Image J?



I am doing confocal fluorescent microscopy of E. coli biofilm.

Is there a way to import nd2 confocal z stack files into image J?

NIS software is propietary, I can use it for taking images, but not in my work computer, I am using the free version of NIS to view my z stack, but I cannot treat the data in any way.

Is there a protocol I can follow to do the zstack in Image J?



Welcome to the forum. The answer is yes! What you need is FIJI ( which is ImageJ with a variety of plugins installed including one called BioFormats which allows FIJI to open a huge number of microscopy proprietary file formats, including .nd2 files.

Good luck.