I aim to measure the length of continuous fluorescent structures from microscopic images in different conditions. I have attached two representative examples. In the first example, the structures are short and relatively straight. In the second, the structures are long and elaborately curved, often spiral-shaped. I have been manually measuring them by drawing an ROI for each structure and then measuring the length (end-to-end distance along the long axis) of the ROI, but I have literally thousands of these structures to measure in several different conditions. Thus, in the interests of both time and objectivity, I was hoping to automate the process. Does anyone have any ideas?
data.zip (1.4 MB)