I’m hoping to get some input/ideas on how to optimize acquisition parameters for image stitching.
In general, I’ve seen that the rule-of-thumb for tiling and stitching is that you want ~20% overlap of the frames (or at least that’s the common default). The downside of 20% overlap (in a grid) is that this means that 64% of your image data is acquired twice.
If you are doing a lot of imaging, say a screen, this can lead to a lot of time acquiring redundant data.
Does anyone have any experience in optimizing the 2D tiling overlap for maintaining stitching accuracy but minimizing the overlap (i.e. decrease imaging time per plate)?
I’m looking for hints/ideas on how to best to tackle this optimization.
-if it matters, I’m using Zeiss-Zen for imaging, which can stitch the images concurrently with acquisition.