Okay, so I really have some problems with tracking my Foci.
First I’ll give you a generelle idea of what I’m hopping to get.
So the data I have is images of a number of Foci within a 2 different Nucleus. My data are first Z-stackes, and then time runs.
So each of my subfolder contains 16 images of the same with different height of the Nucleus, and each subfold is then a different time of the same.
The Foci disappear/appear as a function of Z(Goes out of focus), and as a function of time. What We actually want to measure is how intensity decreases for some Foci and how
some “New” Foci appear and start to increase in intensity.
Since some appear and disappear I use LAP 2nd Phase tracking(since this seems to be the ones I should choose). I can upload my whole Pipeline it that would help.
But here are my trouble with tracking visuallized.
The first image, I don’t really find all foci that are there.
Then I use tracking, which of course just give me this one:
Now the next run is giving me this for identifying Foci:
But now the tracking is very wrong: Foci 1, is not Foci 1 but 6 and so on.