I am trying to set up a Leica DMI4000B microscope (and an Andor camera) with micromanager (to perform some SRRF imaging eventually). I am facing several issues and hopefully someone can help (I don’t know how to code unfortunately).
I was able to follow the instructions on this page: https://micro-manager.org/wiki/LeicaDMI and instructions from Andor to connect the microscope and camera. The only step I skipped is that I didn’t install [Media:Leica-USBDriver.zip] since micromanager can “see” Leica microscope and I believe similar driver has been installed previously (the microscope has already been connected to the PC through a USB cable and can be access using another software).
Everything works fine except for two issues now:
- I can run one-color time-lapse through MDA and the auto shutter is working fine. The shutter is closed between acquisition intervals as expected. However, when I run multi-color time-lapse through MDA, auto shutter is not working properly. The shutter will remain open during the acquisition intervals (I am using “IL shutter” as the “core-shutter”). My feeling is that after the first set of acquisitions for channels 1&2, at the beginning of the acquisition interval, the fluorescence filter turret will move back to the location for channel1 (prepared for the next set of acquisitions) and this turret move activates the IL shutter.
I have submitted a trouble ticket and also hope to get further help from this forum.
- My second issue is on how to control the transmitted light. I tried “TL-shutter” but it is unresponsive. Some early posts stated that Leica DMI4000B actually doesn’t have a “TL-shutter”. Can anyone confirm on this?
Thank you very much for your time and help.