Help in measuring area with the 'wand'

measure
imagej
segmentation

#21

Did you happen to save this macro so that I could access it. If so what did you name it. Thanks


#22

How did you determine that either method was an over or under estimation of the area and not closer to the actual area?

I think this is not the issue here.
From my perspective the more important question is:
What can you expect from a 3D to 2D projection.

This concerns especially the animal legs and I’ve tried to coarsely estimate the maximum area deviation as ±10%. Feel free to tell me your estimated range.

For the sample image, both results deviate for different reasons but the deviations are much smaller than ±10%. My conclusion is that both methods are reasonable because they produce results that are within a range of uncertainty that is independent of the applied method and more or less given by the animal position.

My estimation of the projected animal area of 7.3±0.4cm^2 is from an analysis of the deviations produced by both methods.
Grid-removal: wrongly missing area
Grid-erosion: wrongly included area (left front-leg and shadows)

HTH

Herbie