I am new to CP, and hoping to extract simple size and morphology measurements from organoids, but can seem to get them identified correctly when using either the BF image or 8-bit processed/inverted with imageJ (pics attached)…I know this is super simple, but I’d really appreciate any suggestions on what settings to use in the identify primary objects module. Thanks!!Test.tif (3.0 MB)
You can add other types of measurements and then export your analysis.
If you’re going to use the inverted images, you could uncheck (deactivate) Threshold and MaskImage modules, and follow the steps.
Here are some additional example pipelines:
Hope this helps!
Thanks so much!!! This works beautifully.
Thanks again, really appreciate the help!