I am new to CellProfiler and was trying to use it to find out the ploidy level of nuclei in DAPI stained early stage Drosophila embryos. I am trying to see if the intensity of the nuclei can be related to the ploidy levels. (Something like: more intense the signal more DNA in the nuclei???)
I have a number of 100x100 sq. pixel images that I want to calculate the total fluorescence signal intensity (?), individual nuclei intensities and average nuclear intensity.
Can I get some help with that.