Help aligning plate boundary to binary mask

Hi, I’m trying to adapt the example yeast colony pipeline for my own colony characterization problem and for some reason the binary mask is not aligning to the plate boundary. I have tried all sorts of different settings and for some reason it is still not aligning perfectly. Any suggestions?


Can you provide your pipeline and images so we can have a better look?

Also, did you use the provided template or make your own? I just ask because it looks like the circle is a bit smaller than your pltae.