Hi, I’m trying to process these sort of Images;
I need to determine the edge to edge distance, from both side to side and top to bottom, i.e. i.e i need the longest horizontal and vertical distances of the ellipse (Lens).
I’m not sure how best to achieve this? - I have 1000’s of images to analyse in this manner, any help would be much appreciated!
Hi @Moo
Droplet analysis is by far not a new field, but if you’d like a starting point you can look at the Biomedical Imaging Group at EPFL, they developed two approaches and two ImageJ plugins to assess just what you want. While the batch processing part is non-existent, and manual initializations need to be made, the methods used are pretty powerful.
http://bigwww.epfl.ch/demo/dropanalysis/
Here are a couple of publications as well
Stalder, A F, Melchior, T, Müller, M, Sage, D, Blu, T and Unser, M (2010). Low-Bond Axisymmetric Drop Shape Analysis for Surface Tension and Contact Angle Measurements of Sessile Drops. Coll. Surf. A: Physicochem. Eng. Aspects 364(1–3): 72–81. Software: http://bigwww.epfl.ch/demo/dropanalysis/.
Gassin, P M (2013). Interfaces Liquides/Liquides Actives: Apport de l’Optique Non Linéaire et de la Tensiométrie, PhD Thesis in Chemical Physics. Lyon: Université Claude Bernard. 1 (French). URL: http://tel.archives-ouvertes.fr/tel-00852294.
Gassin, P-M (2014). DROPFIT: a software to compute the interfacial tension, Available at http://www.researchgate.net/publication/262420416_ArchiveDROPFIT_VersionV1.
Best,
Oli
Hi,
I think Bob Dougherty’s measure-ROI-PA plugin will do exactly what you want (measure feret and ortho-feret). It’s availavle from his website: http://www.optinav.com/imagej.html.
bw,
Rob
